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The concentration of Human SIGLEC10 in the samples is then determined by comparing the OD of the samples to the standard curve
Ddit3 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated CTSK antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Sp1
The plate has been pre-coated with Rabbit PIGR antibody
Rat LCN2 (Lipocalin 2) ELISA Kit THBGB1 The concentration of Human SIGLEC10The Rat (LCN2) Lipocalin 2 ELISA Kit measures Lipocalin 2 in Rat samples. The plate has been pre coated with Rat LCN2 antibody. LCN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LCN2 Antibody is added and binds to LCN2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LCN2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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