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Then a biotinylated detection antibody specific for Human CHGA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
IgG1 in samples or Standard competes with a fixed amount of IgG1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IgG1
The plate has been pre-coated with Human SHKBP1 antibody
The Human IL-1Alpha (Interleukin 1 Alpha) ELISA Kit measures IL-1Alpha in Human Serum
NDP present in the sample is added and binds to antibodies coated on the wells
Human ITGA4 (Integrin alpha-4) ELISA Kit PHPTC Then a biotinylated detection antibodyThe Human ITGA4 (Integrin alpha 4) ELISA Kit measures ITGA4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ITGA4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
