C8g (Complement Component 8g) ELISA Kit (Small Sample) H174 and the color intensity is

and the color intensity is directly proportional to the amount of Human CD157 present

Only those wells that contain Mouse SOST

The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve

The OD value is proportional to the concentration of Rat gp130

Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

C8g (Complement Component 8g) ELISA Kit (Small Sample) H174 and the color intensity isThe C8g (Complement Component 8g) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human C8g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human C8g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is