Human CCL11 (Eotaxin) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FABP5

Substrate solution is then added and color develops in proportion to the amount of Human SYNE1

Then a biotinylated detection antibody specific for CHI3L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then Streptavidin-HRP is added and binds to the Biotinylated PPEF1 antibody

it binds with Histone H3 bound to precoated antibody

Human CCL11 (Eotaxin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human CCL11 (Eotaxin) ELISA Kit measures CCL11 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CCL11 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.