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Mouse ACTH in the sample or standard competes with a fixed amount of Mouse ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse ACTH
only those wells that contain Human OPG and HRP-conjugated antibody will exhibit a change in color
then added a HRP-conjugated antibody specific to Mouse PG to each microplate well and incubated
The concentration of Mouse NF-kB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse GROalpha/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mini-sample Mouse SELP (P-Selectin) ELISA Kit PCT Mouse ACTH in the sampleThis P Selectin ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free
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