Human Leptin (LEP) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MCSF

Recombinant Human Osteoprotegerin is produced by our Mammalian expression system and the target gene encoding Glu22-Leu401 is expressed with a 6His tag at the C-terminus

Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

When the sample containing IL- 33 is added to the plate

You can calculate the concentration of Mouse SHH in the samples by comparing the OD of the samples to the standard curve

Human Leptin (LEP) ELISA Kit Packaging:96 wells Standards or samples are addedThis ELISA kit is designed to detect Human Leptin (Human LEP). The assay plate has been pre coated with mouse anti Human Leptin monoclonal antibody. When the sample containing Leptin is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti Human Leptin Antibody is added to the wells and binds to Leptin in the sample. After washing the wells, substrate solutions are added, and the color