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Then Streptavidin-HRP is added and binds to the Biotinylated IL1RAPL2 antibody
Then a biotinylated detection antibody specific for Human BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated ANPEP antibody
The ELAVL1 (ELAV Like Protein 1) ELISA Kit is a Sandwich enzyme immunoassay
The concentration of Human FGF21 in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse OCLN (Occludin) ELISA Kit hIntL Then Streptavidin-HRP is added andThe Mouse (OCLN) Occludin ELISA Kit measures Occludin in Mouse samples. The plate has been pre coated with Mouse OCLN antibody. OCLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse OCLN Antibody is added and binds to OCLN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OCLN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
