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Then a biotinylated detection antibody specific for Rat CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated Serpina3N antibody
Calculate the concentration of PTX3/Pentraxin 3 in the sample by plotting standard curve
it binds with HNF4a bound to precoated antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse EDN2
Rat FBP1 (Fructose-1,6-bisphosphatase 1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat FBP1 (Fructose 1,6 bisphosphatase 1) ELISA Kit measures Fbp1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti FBP1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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