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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL1a2
Then Streptavidin-HRP is added and binds to the Biotinylated EGLN1 antibody
You can calculate the concentration of Mouse GzmB in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human TGF-Beta3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
HIF-1Alpha Mouse Monoclonal Antibody (Mix-mA)
Mouse FSH (Follicle-stimulating Hormone) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Mouse (FSH) Follicle stimulating Hormone ELISA Kit measures follicle stimulating hormone in Mouse samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB
