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it is eluted with RNase-Free ddH2O
and the color intensity is directly proportional to the amount of Human C7 present
And then biotinylated Bovine F11R Antibody is added and binds to F11R in the sample
FER present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated DCR3 antibody
Mouse PARP1 (Poly) ELISA Kit PC-I it is eluted with RNase-FreeThe Mouse (PARP1) Poly ELISA Kit measures Poly in Mouse samples. The plate has been pre coated with Mouse PARP1 antibody. PARP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PARP1 Antibody is added and binds to PARP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PARP1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.