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A standard curve is prepared relating color intensity to the concentration of 17-OHP
Then Streptavidin-HRP is added and binds to the Biotinylated PREX2 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated GALR1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human TPT1
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL18BP
Mouse Flt3L (FMS-like Tyrosine Kinase 3 Ligand) ELISA Kit LGALS1 A standard curve is preparedThe Mouse Flt3L (FMS like Tyrosine Kinase 3 Ligand) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse Flt3L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Flt3L and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
