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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD5L
The plate has been pre-coated with Canine HMGB1 antibody
You can calculate the concentration of DAO in the samples by comparing the OD of the samples to the standard curve
And then biotinylated Porcine CD163 Antibody is added and binds to CD163 in the sample
Substrate solution is then added and color develops in proportion to the amount of Human ABCC1
Human CLDN6 (Claudin 6) ELISA Kit IL-6RB Standards or samples are addedThe Human CLDN6 (Claudin 6) ELISA Kit measures CLDN6 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CLDN6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
