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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPARa
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken Pg are added to the micro ELISA plate wells
it binds with CD86 bound to precoated antibody
And then biotinylated Human GTPBP1 Antibody is added and binds to GTPBP1 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated CLRN1 antibody
Bovine LEP (Leptin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Bovine LEP (Leptin) ELISA Kit measures Lep in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Lep antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
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