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The concentration of Human FGF21 in the samples is then determined by comparing the OD of the samples to the standard curve
You can calculate the concentration of Mouse TRAIL/TNFSF10 in the samples by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse VEGF-A
Then a biotinylated detection antibody specific for Rat IgG2b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then a biotinylated detection antibody specific for Human F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Equine IFN-beta ELISA Kit Packaging:96 wells The concentration of Human FGF21The Equine IFN beta ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Horse IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Horse IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
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