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Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Human TACE/ADAM17 (TNF Alpha Converting Enzyme) ELISA kit uses the Sandwich-ELISA principle
Only those wells that contain Mouse IgG
The Mouse ACTAlpha2 (Actin Alpha 2
and the yield is generally about 100-400ug
Beta-Tubulin Mouse mAb IL37 Then a biotinylated detection antibodyMicrotubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha and beta tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of Beta Tubulin may not be stable in certain cells. For example, expression of Beta Tubulin in adipose tissue is very low
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