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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NEFL
The concentration of IL12 in the samples is then determined by comparing the OD of the samples to the standard curve
APOD present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated CLDN20 antibody
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine E3 are added to the micro ELISA plate wells
Rat PROKR1 (Prokineticin Receptor 1) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Rat (PROKR1) Prokineticin Receptor 1 ELISA Kit measures Prokineticin Receptor 1 in Rat samples. The plate has been pre coated with Rat PROKR1 antibody. PROKR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PROKR1 Antibody is added and binds to PROKR1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PROKR1 antibody. After incubation unbound Streptavidin HRP is washed
