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The concentration of Sheep IL4 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT33A
The concentration of Human BMPER in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse IgG2b
Human MT in the sample or standard competes with a fixed amount of Human MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MT
HCAM (Homing Associated Cell Adhesion Molecule) ELISA Kit (Small Sample) Packaging:48 wells The concentration of Sheep IL4The HCAM (Homing Associated Cell Adhesion Molecule) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human HCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
