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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1a
The plate has been pre-coated with Bovine LUM antibody
and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately
Only those wells that contain Human IL-1ra/IL-1F3
The concentration of Rat CR in the samples is then determined by comparing the OD of the samples to the standard curve
Canine F9 (Coagulation Factor 9) ELISA Kit NL2 Standards or samples are addedThe Canine (F9) Coagulation Factor 9 ELISA Kit measures Coagulation Factor 9 in Canine samples. The plate has been pre coated with Canine F9 antibody. F9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine F9 Antibody is added and binds to F9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated F9 antibody. After incubation unbound Streptavidin HRP is washed away during a
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