Rat IL-3 (Interleukin 3) ELISA Kit Packaging:96 wells The plate has been pre-coated

The plate has been pre-coated with Rat MMP-13 antibody

Then Streptavidin-HRP is added and binds to the Biotinylated AMY2A antibody

Then a biotinylated detection antibody specific for Rat IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFB

only those wells that contain Human Hepc 25

Rat IL-3 (Interleukin 3) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Rat (IL 3) Interleukin 3 ELISA Kit measures Interleukin 3 in Rat samples. The plate has been pre coated with Rat IL 3 antibody. IL 3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IL 3 Antibody is added and binds to IL 3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL 3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.