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And then biotinylated Bovine IL4 Antibody is added and binds to IL4 in the sample
And then biotinylated Mouse TP63 Antibody is added and binds to TP63 in the sample
Calculate the concentration of C4B in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated SKI antibody
The plate has been pre-coated with Human KRT1 antibody
Rat C3 (Complement Component 3) ELISA Kit SERPING1 And then biotinylated Bovine IL4The Rat C3 (Complement Component 3) ELISA Kit measures C3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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