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Then a biotinylated detection antibody specific for Mouse GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
CCNB1 present in the sample is added and binds to antibodies coated on the wells
You can calculate the concentration of Mouse PSA in the samples by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human PITPNC1
And then biotinylated Human EFNB2 Antibody is added and binds to EFNB2 in the sample
Human anti-GBM (Anti glomerular basement membrane antibody) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human anti GBM (Anti glomerular basement membrane antibody) ELISA Kit measures GBM Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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