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Substrate solution is then added and color develops in proportion to the amount of Human ATG12
Then Streptavidin-HRP is added and binds to the Biotinylated OSBPL10 antibody
You can calculate the concentration of Human Zonulin in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated FAM83D antibody
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Human NTN4 (Netrin-4) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human NTN4 (Netrin 4) ELISA Kit measures NTN4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NTN4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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