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Only those wells that contain Rat bFGF/FGF2
and the color intensity is directly proportional to the amount of Mouse TNFR-2 present
Then a biotinylated detection antibody specific for Human C1INH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Rat CLCF1
The concentration of Human FCN2 in the samples is then determined by comparing the OD of the samples to the standard curve
COL4 (Collagen Type IV) ELISA Kit (Small Sample) Hepapoietin Only those wells that containThe COL4 (Collagen Type IV) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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