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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOM
And then biotinylated Human ADRA2A Antibody is added and binds to ADRA2A in the sample
and the color intensity is directly proportional to the amount of Human NGF present
The Rat CCL9/MIP1g ELISA kit is a sandwich enzyme immunoassay
Then Streptavidin-HRP is added and binds to the Biotinylated APCDD1 antibody
Rat AQP-1 (Aquaporin 1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (AQP 1) Aquaporin 1 ELISA Kit measures Aquaporin 1 in Rat samples. The plate has been pre coated with Rat AQP1 antibody. AQP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AQP1 Antibody is added and binds to AQP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AQP1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.