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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOO
And then biotinylated Human LEFTY2 Antibody is added and binds to LEFTY2 in the sample
And then biotinylated Rat SLC15A1 Antibody is added and binds to SLC15A1 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated S100A6 antibody
Substrate solution is then added and color develops in proportion to the amount of Rat IL10
Human KLF15 (Kruppel Like Factor 15) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (KLF15) Kruppel Like Factor 15 ELISA Kit measures Kruppel Like Factor 15 in samples. The plate has been pre coated with Human KLF15 antibody. KLF15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KLF15 Antibody is added and binds to KLF15 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KLF15 antibody. After incubation unbound Streptavidin HRP is washed away
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