Hamster TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit NAP2 only those wells that contain

only those wells that contain Mouse GAL12

And then biotinylated Rabbit TIMP2 Antibody is added and binds to TIMP2 in the sample

Then a biotinylated detection antibody specific for Rat NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

and the color intensity is directly proportional to the amount of Human Cystatin F present

Substrate solution is then added and color develops in proportion to the amount of Mouse IL1B

Hamster TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit NAP2 only those wells that containThe Hamster TIMP 2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit measures TIMP 2 in Hamster Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TIMP 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.