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You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse GROBeta/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated SLC30A7 antibody
The plate has been pre-coated with Human CENPM antibody
Only those wells that contain Human GM-CSF
Human CFN (Cellular Fibronectin) QuickTest ELISA Kit CDw121b You can calculate the concentrationThe Human CFN (Cellular Fibronectin) QuickTest ELISA Kit measures cFN in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
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