Human COL4 (Collagen Type IV) high sensitivity ELISA Kit PEM Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Sheep IL-1alpha

Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTS2 antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EPO

And then biotinylated Human BPI Antibody is added and binds to BPI in the sample

Calculate the concentration of AMD1 in the sample by plotting standard curve

Human COL4 (Collagen Type IV) high sensitivity ELISA Kit PEM Substrate solution is then addedThe Human COL4 (Collagen Type IV) high sensitivity ELISA Kit measures COL4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti COL4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated