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You can calculate the concentration of Human sIgA in the samples by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human MIP-1α
The OD value is proportional to the concentration of Mouse IL-4
Then a biotinylated detection antibody specific for Human IL-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-kB-p65
GAP43 Mouse mAb NAP3 You can calculate the concentrationGrowth Associated Protein 43 also known as GAP43 is a protein that in humans is encoded by the GAP43 gene. GAP43, is a nervous tissue specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts.