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Then a biotinylated detection antibody specific for Mouse KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
You can calculate the concentration of Mouse MCP-2 in the samples by comparing the OD of the samples to the standard curve
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The OD value is proportional to the concentration of Mouse IL-4
The kit can also be used for gene-specific reverse transcription
Multi-Species Prostaglandin D2 ELISA Kit RT-PCR Then a biotinylated detection antibodyThe Prostaglandin D2 ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal PGD2. During the reaction, Universal PGD2 in the sample or standard competes with a fixed amount of Universal PGD2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGD2. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish
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