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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig LEP
Only those wells that contain Rat IgM
a horseradish peroxidase conjugated mouse anti-Human DOPA Decarboxylase Antibody is added to the wells and binds to DOPA Decarboxylase in the sample
The concentration of Human FETUA in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat gp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
BRCC3 (BRCA1/BRCA2 Containing Complex Subunit 3) ELISA Kit (Small Sample) Packaging:48 wells Standards or samples are addedThe BRCC3 (BRCA1 BRCA2 Containing Complex Subunit 3) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human BRCC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human BRCC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
