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RNase protection analysis
Recombinant Human Pro-Neuropeptide Y is produced by our Mammalian expression system and the target gene encoding Tyr29-Trp97 is expressed with a 6His tag at the C-terminus
The concentration of Human Lpa in the samples is then determined by comparing the OD of the samples to the standard curve
Recombinant Human Membrane Primary Amine Oxidase is produced by our Mammalian expression system and the target gene encoding Arg28-Asn763 is expressed with a Fc tag at the C-terminus
EnTurbo™+ SYBR Color qPCR SuperMix is an optimized 2 x real-time PCR master mix containing HotStar aq DNA Polymerase
EasyStep Human MMP-8 (Matrix Metalloproteinase-8) ELISA Kit Packaging:96 wells + 16 wells RNase protection analysisEach EasyStep ELISA kit comes with two extra strips of coated wells to run for standard curves ensuring you have more wells for your samples. The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MMP 8, and the Human MMP 8 standard plate wells that pre coated using protein related techniques are provided separately. Standard Sample Diluent
