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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALT
And then biotinylated Feline IL-8 Antibody is added and binds to IL-8 in the sample
dNTP and Mg2+
Then Streptavidin-HRP is added and binds to the Biotinylated GPS1 antibody
You can calculate the concentration of Human HPA in the samples by comparing the OD of the samples to the standard curve
Rat CYS-C (Cystatin C) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (CYS C) Cystatin C ELISA Kit measures Cystatin C in Rat samples. The plate has been pre coated with Rat CST3 antibody. CST3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CST3 Antibody is added and binds to CST3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CST3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
