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Then a biotinylated detection antibody specific for CD48 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated ACACB antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OxLDL
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC1A5
Then Streptavidin-HRP is added and binds to the Biotinylated ANXA3 antibody
Rat MDK (Midkine) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat (MDK) Midkine ELISA Kit measures Midkine in Rat samples. The plate has been pre coated with Rat MDK antibody. MDK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MDK Antibody is added and binds to MDK in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MDK antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution is
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