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Then a biotinylated detection antibody specific for Human sTfR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTACK
coli expression system and the target gene encoding Asp89-Leu235 is expressed
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1ra/IL-1F3
Rat IgG1 (Immunoglobulin G1) ELISA Kit TFR1 Then a biotinylated detection antibodyThis Rat IgG1 (Immunoglobulin G1) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with IgG1. During the reaction, IgG1 in samples or Standard competes with a fixed amount of IgG1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IgG1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish
