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Substrate solution is then added and color develops in proportion to the amount of Rat ODC1
If the sample contains CCL6
FNBP1 present in the sample is added and binds to antibodies coated on the wells
Human IgG2 in the sample or standard competes with a fixed amount of Human IgG2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human IgG2
a capture antibody-BTN2A1-biotin-detection antibody complex was formed
Guinea pig C5a (Complement Component 5a) ELISA Kit B2MG Substrate solution is then addedThe Guinea pig C5a (Complement Component 5a) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig C5a and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
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