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Then a biotinylated detection antibody specific for Human CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human GPC4 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated Renin antibody
The OD value is proportional to the concentration of Mouse ACTα2
You can calculate the concentration of Human TGF-Beta2 in the samples by comparing the OD of the samples to the standard curve
Human MDH2 (Malate dehydrogenase, mitochondrial) QuickTest ELISA Kit PTC Then a biotinylated detection antibodyThe Human MDH2 (Malate dehydrogenase, mitochondrial) QuickTest ELISA Kit measures MDH2 in Human Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the