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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SYN1
The concentration of Human RGS19 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL22
Then a biotinylated detection antibody specific for Human SYNPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Rat CD36
Rat PCX (Podocalyxin) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (PCX) Podocalyxin ELISA Kit measures Podocalyxin in Rat samples. The plate has been pre coated with Rat PODXL antibody. PODXL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PODXL Antibody is added and binds to PODXL in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PODXL antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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