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then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated
The plate has been pre-coated with Rat CA3 antibody
Substrate solution is then added and color develops in proportion to the amount of Rat GGPS1
Then Streptavidin-HRP is added and binds to the Biotinylated TP73 antibody
The GRAP2 (GRB2 Related Adaptor Protein 2) ELISA Kit is a Sandwich enzyme immunoassay
Rat IgM (Immunoglobulin M) ELISA Kit Packaging:96 wells then added a HRP-conjugated antibodyThe Rat (IgM) Immunoglobulin M ELISA Kit measures Immunoglobulin M in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and
