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The COL4a5 (Collagen Type IV Alpha 5) ELISA Kit is a Sandwich enzyme immunoassay
Sheep Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol
Then Streptavidin-HRP is added and binds to the Biotinylated STAT6 antibody
It is a Competitive ELISA kit
Substrate solution is then added and color develops in proportion to the amount of Human ELOVL2
Human PITX2 (Pituitary Homeobox 2) ELISA Kit Proto-oncogene tyrosine-protein kinase receptor Ret The COL4a5 (Collagen Type IVThe Human (PITX2) Pituitary Homeobox 2 ELISA Kit measures Pituitary Homeobox 2 in samples. The plate has been pre coated with Human PITX2 antibody. PITX2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PITX2 Antibody is added and binds to PITX2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PITX2 antibody. After incubation unbound Streptavidin HRP is washed away
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