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The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Galectin-3 in the specimen
Then a biotinylated detection antibody specific for Human COL1Alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microplate provided in this kit has been precoated with anti DTWD1 antibody
Calculate the concentration of FG in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Human FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat Gliadin-IgM (Gliadin Immunoglobulin M) ELISA Kit Packaging:96 wells The absorbance values are measured#VALUE!
