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Human CASP3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CASP3
The plate has been pre-coated with Bovine MB antibody
Then Streptavidin-HRP is added and binds to the Biotinylated DCTN5 antibody
And then biotinylated Human DEFA6 Antibody is added and binds to DEFA6 in the sample
Calculate the concentration of Sgk1 in the sample by plotting standard curve
Rat REG3B (RegeneRating islet-derived protein 3-beta) QuickTest ELISA Kit Packaging:96 wells Human CASP3 in samples (orThe Rat REG3B (RegeneRating islet derived protein 3 beta) QuickTest ELISA Kit measures Reg3b in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and
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