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The plate has been pre-coated with Human APOD antibody
which are expressed on the surfaces of other cells
The concentration of Human HRH4 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Feline IL-1B antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAX6
Rat NT-4 (Neurotrophin 4) ELISA Kit TRF The plate has been pre-coatedThe Rat (NT 4) Neurotrophin 4 ELISA Kit measures Neurotrophin 4 in Rat samples. The plate has been pre coated with Rat NT 4 antibody. NT 4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT 4 Antibody is added and binds to NT 4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NT 4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
