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And then biotinylated Rat NES Antibody is added and binds to NES in the sample
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of α-Endorphin in the sample
it binds with HSP GP96 bound to precoated antibody
The concentration of Mouse MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve
Calculate the concentration of C3b in the sample by plotting standard curve
Rat SCD100 (soluble Cluster of differentiation 100) QuickTest ELISA Kit Packaging:96 wells And then biotinylated Rat NESThe Rat SCD100 (soluble Cluster of differentiation 100) QuickTest ELISA Kit measures sCD100 in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and
