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And then biotinylated Human CHI3L2 Antibody is added and binds to CHI3L2 in the sample
Calculate the concentration of PION in the sample by plotting standard curve
The concentration of VNN1 in the sample was calculated by drawing a standard curve
The plate has been pre-coated with Human CDK1 antibody
Then a biotinylated detection antibody specific for Rat PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rabbit E2 (Estradiol) ELISA Kit Packaging:96 wells And then biotinylated Human CHI3L2The Rabbit E2 (Estradiol) ELISA Kit measures E2 in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with E2. E2 in the sample or standard competes with a fixed amount of biotin labeled E2 for sites on a pre coated antibody specific to E2. Free components are washed off. After adding HRP
