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Then Streptavidin-HRP is added and binds to the Biotinylated FSHR antibody
Substrate solution is then added and color develops in proportion to the amount of Human H3C1
only those wells that contain Human NFASC
You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human C/EBPALPHA (CCAAT/Enhancer Binding Protein Alpha) ELISA Kit INDO Then Streptavidin-HRP is added andThe Human C EBPALPHA (CCAAT Enhancer Binding Protein Alpha) ELISA Kit measures C EBPAlpha in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C EBP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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