Human PGII (Pepsinogen II) ELISA Kit acetylhydrolase Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human BTNL8

ITGAL present in the sample is added and binds to antibodies coated on the wells

and the absorbance is measured spectrophotometrically at 450nm

Then a biotinylated detection antibody specific for Mouse CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The OD value is proportional to the concentration of Human IP-10/CXCL10

Human PGII (Pepsinogen II) ELISA Kit acetylhydrolase Substrate solution is then addedThe Human PGII (Pepsinogen II) ELISA Kit measures PGII in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PGII antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.