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Substrate solution is then added and color develops in proportion to the amount of Human CALB1
Then a biotinylated detection antibody specific for PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
FCN3 present in the sample is added and binds to antibodies coated on the wells
The Human CXCL14/BRAK ELISA kit is a sandwich enzyme immunoassay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHGA
Rat CT-1 (Cardiotrophin 1) ELISA Kit Proto-oncogene c-Ret Substrate solution is then addedThe Rat (CT 1) Cardiotrophin 1 ELISA Kit measures cardiotrophin 1 in Rat samples. The plate has been pre coated with Rat CT 1 antibody. CT 1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CT 1 Antibody is added and binds to CT 1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CT 1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
