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Substrate solution is then added and color develops in proportion to the amount of Human PPP1R8
it binds with PRSS36 bound to precoated antibody
it binds with SNX1 bound to precoated antibody
Calculate the concentration of NMNAT1 in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RGN
Mouse RGN (Regucalcin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse RGN (Regucalcin) ELISA Kit measures RGN in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti RGN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
