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When the sample containing BCAM is added to the plate
Then Streptavidin-HRP is added and binds to the Biotinylated C5 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated BDNF antibody
Substrate solution is then added and color develops in proportion to the amount of Chicken IL-18
Then a biotinylated detection antibody specific for Rat IgG2c and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human OTUB2 (Ubiquitin Thioesterase Otub2) ELISA Kit Packaging:96 wells When the sample containing BCAMThe Human (OTUB2) Ubiquitin Thioesterase Otub2 ELISA Kit measures Ubiquitin thioesterase OTUB2 in samples. The plate has been pre coated with Human OTUB2 antibody. OTUB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human OTUB2 Antibody is added and binds to OTUB2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OTUB2 antibody. After incubation unbound Streptavidin HRP is
